June 17, 2016

Microorganisms can eat plastic, and it could save the Earth (or almost) - by Dara

Microorganisms can eat plastic, and it could save the Earth
(or almost)

A taste of Dara Nguyen & Paul-Henry’s review:


Each year, 250 million tons of plastic are produced but only 8% of it is recycled. A large part of it is released in nature, mostly in soils and in the oceans, thus highly disrupting ecosystems. In particular, the ingestion of small plastic fragments can cause wildlife cancers, and big fragments can lead to malformations (see picture below).


Plastics, that are polymers, are not easy to degrade because the strong bonds linking the monomers, which gives it interesting properties, are hard to break. Different methods exist, but it seems that there is no efficient and eco-friendly way to take care of these wastes. A new approach is being developed using the ability of some microorganisms to digest polymers.


What are these microorganisms?
What kind of plastics can they digest?


For this review, we did a bibliography of articles dealing with plastic-degrading microorganisms and tried to see the connections between them: how similar and different the mentioned microorganisms are, according to what criteria? What families of plastics are concerned? What can we deduce on the enzymatic mechanism implied? As we expected, we observed a large variety of species and polymers. We mainly focused on this diversity. Indeed, if several microorganisms can digest several polymers, it makes it a better weapon against the vast enemy that is plastic waste.


Our work could be summarized with the figure below. The most common classes of plastic polymers are represented with an example on the left. Microorganisms which can attack them are linked with a green dash. Else if we could not find any species that can deal with this class, it is followed by a red dash. As for the mechanism, they are not accurately identified and they are not the same for all species, so they do not appear on this figure. We can already see the diversity of polymer structure with their chemical formula; as for the microorganisms, you will find more details in the review and especially in the attached references (in brackets on the figure).



Our main conclusions are that plastic-digesting microorganisms can be bacteria or fungus, from any habitat, but they are all saprophytic – which seems obvious, saprophytic meaning that they can feed on non-living organic matter). As a consequence, they can attack several organic polymers although not all of them, but they have some trouble dealing with inorganic polymers. Maybe we could understand why some organisms and not others, why some plastics and not others, by better understanding the mechanisms. Studying and modeling this process could open the way to technologies that function the same and that we would control better.



If you want to know more…

TED talk: “Two young scientists break down plastics with bacteria
  
[1]           greenhome.com, 2016. The life of a plastic bottle [online]. Available at:
[2]           Moore, C. J., 2008. Synthetic polymer in the marine environment: A rapidly increasing, long-term threat. Environmental Research. 108, 131-139.     
[3]           Pathma, J., Sakthivel, N., 2012. Microbial diversity of vermicompost bacteria that exhibit useful agricultural traits and waste management potential. SpringerPlus. 1:26.
[4]           Stloukal, P., Pekarova, S., Kalendova, A., Mattausch, H., Laske, S., Holzer, C., Chitu, L., Bodner, S., Maier, G., Slouf, M., Koutny, M., 2015. Kinetics and mechanism of the biodegradation of PLA/clay nanocomposites during thermophilic phase of composting process. Waste Management. 42, 31–40.
[5]           Morét-Ferguson, S., Lavender, K., Proskurowski, G., Murphy, E. K., Peacock, E. E., Reddy, C. M., 2010. The size, mass, and composition of plastic debris in the western North Atlantic Ocean. Marine Pollution Bulletin. 60, 1873–1878.
[6]           Nakkabi, A., Sadiki, M., Ittobane, N., Ibnsouda Koraichi, S., Barkai, H., El Abed, S.,2015. Biodegradation of Poly(ester urethane)s by Bacillus subtilis. International Journal of Environmental Research. 9, 157-62.
[7]           Nakkabi, A., Sadiki, M., Koraichi Saad, I., Fahim, M., 2015. Biological degradation of polyurethane by a newly isolated wood bacterium. International Journal of Recent Advanced in Multidisciplinary Research. 2, 222-225.
[8]           Delafield, F. P., Doudoroff, M., Palleroni, N. J., Lusty, C. J., Contopoulos, R., 1965. Decomposition of poly-β-hydroxybutyrate by Pseudomonads. Journal of Bacteriology. 90, 1455-1466.
[9]           Yang, Y., Yang, J., Wu, W. M., Zhao, J., Song, Y., Gao, L., Yang, R., Jiang, L., 2015. Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 2. Role of gut microorganisms. Environmental Science and Technology. 49, 12087-93.

[10]        Espinosa-Valdemar, R. M., Turpin-Marion, S., Delfin-Alcala, I., Vazquez-Morillas, A., 2011. Disposable diapers biodegradation by the fungus Pleurotus Ostreatus. Waste Management. 31, 1683-8.

[11]        Shinozaki, Y., Morita, T., Cao, X. H., Yoshida, S., Koitabashi, M., Watanabe, T., Suzuki, K, Sameshima-Yamashita, Y., Nakajima-Kambe, T., Fujii, T., Kitamoto, H. K., 2013. Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization. Applied Microbiology and Biotechnology. 97, 2951–2959.
[12]        Tsujiyama, S., Okada, A., 2013. Biodegradation of polyvinyl alcohol by a brown-rot fungus, Fomitopsis pinicola. Biotechnology Letters. 35, 1907-11.
[13]        Santo, M., Weitsman, R., Sivan, A., 2013. The role of the copper-binding enzyme – laccase – in the biodegradation of polyethylene by the actinomycete Rhodococcus ruber. International Biodeterioration & Biodegradation. 84, 204-210.
[14]        Mohan, A. J., Sekhar, V. C., Bhaskar, T., Nampoothiri, K. M., In press. Microbial assisted High Impact Polystyrene (HIPS) degradation. Bioresource Technology.

[15]        Ishigaki, T., Sugano, W., Ike, M., Kawagoshi, Y., Fukunaga, I., Fujita, M., 2000. Abundance of polymers degrading microorganisms in a sea-based solid waste disposal site. Journal of Basic Microbiology. 40, 177-186.

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